Janssen, J.J.M., Bovee-Geurts, P.H.M., Merkx, M. and DeGrip, W.J. (1995) Histidine-tagging both allows convenient single-step purification of bovine rhodopsin and exerts ionic-strength dependent effects on its photochemistry. J. Biol. Chem. 270, 11222-11229.

DeGrip, W.J., VanOostrum, J. and Bovee-Geurts, P.H.M. (1998) Selective detergent-extraction from mixed detergent/lipid/protein micelles, using cyclodextrin inclusion compounds: A novel generic approach for the preparation of proteoliposomes. Biochem. J. 330, 667-674.

Bosman, G.J.C.G.M., Van Oostrum, J., Breikers, G., Bovee-Geurts, P.H.M., Klaassen, C.H.W., DeGrip, W.J. (2003) Functional expression of His-tagged rhodopsin in Sf9 insect cells. in Methods in Molecular Biology; Membrane Protein Protocols, Ed. Selinsky, B.S. p. 73-88

Stable isotope labelling of biomolecules

Egorova, T.A., Eremin, S.V., Mitsner, B.I., Zvonkova, E.N., Shvets, V.I. (1995) Isolation of individual amino acids from various microbiological sources using reversed-phase high performance liquid chromatography. Journal of Chromatography, Biomedical Application 665, 53-62.

Egorova-Zachernyuk, T.A., Shvets V.I., Versluis K., Heerma, W., Creemers, A.F.L., Nieuwenhuis, S.A.M., Lugtenburg, J., Raap, J. (1996) Preparation of site-specifically labelled zervamicins, the antibiotic peptaibols produced by Emericellopsis salmosynnemata. Journal of Peptide Science. 2, 1-10.

Protein structure

Egorova-Zachernyuk, T.A., Hollander, J., Frazer N., Gast, P., Cogdell, R., Hoff, A.J., De Groot, H.J.M., and Baldus, M. (2001) Backbone correlations in a fully labelled membrane protein complex at ultra high magnetic fields. J. Biomol. NMR, 19, 243-253

Ligand - protein interaction

Egorova-Zachernyuk T.A., (1997) NMR Characterization of Protein-Pheophytin Interactions in Rhodobacter sphaeroides R-26 Photosynthetic Reaction Centers, from multispin pheophytin enrichment and 2-D MAS NMR dipolar correlation spectroscopy. Biochemistry 36, 7513-7519.

Egorova-Zachernyuk T.A., Remy A., Shkuropatov A.Ya., Gast P., Hoff A.J., Gerwert K., de Groot H.J.M. (1999) Efficient conditions for the photoaccumulation of HA - in the photosynthetic reaction centers of Rhodobacter sphaeroides R-26 with uniformly labelled bacteriopheophytin monitored by fourier transform infrared difference spectroscopy. Vibrational spectroscopy, 19, 347-352.

Creemers, A.F.L., Klaassen, C.H.W., Bovee-Geurts, P.H.M., Kelle, R., Kragl, U., Raap, J., DeGrip, W.J., Lugtenburg, J. and DeGroot, H.J.M. (1999) 15N Solid state NMR evidence for a compex Schiff base counterion in the visual G-protein coupled receptor rhodopsin. Biochemistry -USA 38, 7195-7199.

Creemers A.F.L., Kiihne S., Bovee-Geurts P.H.M., de Grip W.J., Lugtenburg, J., and de Groot H.J.M. (2002). 1H and 13C MAS NMR evidence for pronounced ligand-protein interactions involving the ionone ring of the retinylidene chromophore in rhodopsin. Proc. Nat. Acad. Sci. 99, 9101-9106

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